SAQPhysiologyNeurophysiologyOctober 2013 · Pain pathway and modulation

Question bank · October 2013 · Physiology

One word decides the definition mark,
and part (b) is not answerable in prose alone.

Show the model answerAttempt it first — that is what makes it stick

(a) Define pain 1 mark

Commonly lost: The commonest error was omitting “potential” tissue damage, the word that accommodates pain with no demonstrable injury. Dropping “emotional” reduces pain to nociception.

TermDefinitionCan occur without the other
NociceptionThe neural process of encoding a noxious stimulusYes: it occurs under anaesthesia with no pain
PainThe conscious experienceYes: central and neuropathic pain occur without nociception

(b) The afferent pain pathway 5 marks

What earns the marks5 marks

A diagramRequired, not optional: some answers omitted it
TransductionNociceptors: free nerve endings; inflammatory mediators lower threshold
The two fibresAδ = first, fast, sharp; C = second, slow, dull
First order neuronCell body in the dorsal root ganglion → synapse in the dorsal horn
Second order neuronDorsal horn → decussates → contralateral spinothalamic tract → thalamus
Third order neuronThalamus → internal capsule → somatosensory cortex and limbic areas

Commonly lost: The question said “describe and illustrate. A diagram was required and some answers omitted it entirely.

Pathway

The ascending pathway, in three neurons

Read it from the bottom. The first-order neuron has its cell body in the dorsal root ganglion, outside the cord, and synapses in the dorsal horn. The second-order neuron starts in the dorsal horn, crosses in the cord and ascends on the opposite side. At the brainstem the projection divides: the lateral, neospinothalamic route relays in the ventral posterolateral nucleus (VPL) and reaches the primary somatosensory cortex, which is where and how intense; the medial, paleospinothalamic route passes through the reticular formation and periaqueductal grey to the intralaminar nuclei and on to the cingulate and insula, which is how unpleasant. The third-order neuron runs from thalamus to cortex. From the face the plan is the same, with the first-order cell body in the trigeminal ganglion, the second in the spinal trigeminal nucleus and the third in the ventral posteromedial nucleus.

CortexS1, S2, insula, anterior cingulateThalamusVPL (lateral) and intralaminar (medial)BrainstemReticular formation, periaqueductal greySpinal cordDorsal horn, laminae I, II and VPeripheryFree nerve endings of nociceptorsmidlineDorsal root ganglionfirst-order cell bodyDorsal horncrosses hereReticular formationand PAGVPLIntralaminarS1: locationCingulate, insula: affect123NeospinothalamicPaleospinothalamic

1 · Transduction: the receptor

  • Nociceptors are free (bare) nerve endings in skin, muscle, joint, periosteum and viscera.
  • Activated by mechanical, thermal or chemical stimuli of intensity sufficient to threaten tissue.
  • Threshold lowered by inflammatory mediators: bradykinin, prostaglandins, histamine, serotonin, K⁺, H⁺, substance P.

2 · The two fibre types

C
MyelinationThinly myelinatedUnmyelinated
Diameter2-5 µm0.4-1.2 µm
Conduction velocity~12-30 m/s~0.5-2 m/s
ModalityMechanical and thermal nociceptionPolymodal
Pain carriedFirst (fast) painSecond (slow) pain
CharacterSharp, pricking, well localised, rapid onset and offsetDull, burning, aching, poorly localised, persistent
Dorsal horn laminaI, and more deeply VII (substantia gelatinosa)

3 · The three orders of neuron

OrderCell bodyCourseTerminates
FirstDorsal root ganglionPseudounipolar: peripheral process to the nociceptor, central process entering via the lateral division of the dorsal root, branching in the tract of Lissauer over 2-3 segmentsDorsal horn, laminae I, II and V. Transmitters glutamate and substance P
SecondDorsal hornDecussates in the anterior white commissure within one or two segments; ascends contralaterally in the anterolateral quadrant as the spinothalamic tractThalamus: VPL (lateral division) and intralaminar/medial nuclei (medial division)
ThirdThalamusThrough the posterior limb of the internal capsuleS1 and S2 in the postcentral gyrus (from VPL); insula and anterior cingulate (from medial nuclei)

Commonly lost: The dorsal root ganglion (outside the cord, holding the first order cell body) was confused with the dorsal horn (grey matter inside the cord, where that neuron synapses).

4 · The two divisions of the tract

Lateral (neospinothalamic)Medial (paleospinothalamic)
Thalamic relayVentral posterolateral (VPL) nucleusIntralaminar and medial nuclei
Cortical targetPrimary and secondary somatosensory cortexInsula, anterior cingulate, limbic structures
CarriesWhere and how much: location, intensity, qualityHow unpleasant: affect, arousal, autonomic response
CollateralsFewReticular formation, periaqueductal grey
Corresponds toThe sensory half of the definition in (a)The emotional half of the definition in (a)

Because the fibres cross close to their level of entry, cord hemisection gives loss of pain and temperature contralateral to the lesion with loss of proprioception ipsilateral, the dissociation that identifies the tract.

(c) How the perception of pain may be modified 4 marks

What earns the marks4 marks

Gate control theoryMelzack and Wall; required to pass
Large and small fibresTheir opposite effects on the inhibitory interneuron
Descending inhibitionPAG → RVM/raphe and locus coeruleus → dorsal horn. Also required to pass
MediatorsSerotonin, noradrenaline, endogenous opioids; additional marks
OtherPsychological, peripheral and pharmacological modification

Commonly lost: Both gate control and the descending inhibitory pathway were needed to pass. One without the other did not.

1 · Gate control theory (Melzack and Wall, 1965)

Circuit

The gate, drawn as a circuit

Solid lines excite (+); dashed lines inhibit (−). Both afferents excite the projection neuron directly. The difference is what each does to the inhibitory interneuron in the substantia gelatinosa: the large Aβ fibre excites it, so touch closes the gate; the small C fibre inhibits it, so a noxious input removes the brake and opens the gate twice over. Descending fibres close it from above, by exciting the interneuron and by inhibiting the projection neuron directly.

PAG, RVM and locus coeruleusDescending+descendingAβ fibrelarge, myelinated, touchC fibresmall, unmyelinated, nociceptive+++SGProjTo the brainspinothalamic tractSG: inhibitory interneuron, substantia gelatinosa (lamina II)Proj: projection neuron

Transmission from first to second order neuron in the substantia gelatinosa is not obligatory: an inhibitory interneuron, when active, closes the gate to the projection neuron.

InputEffect on the inhibitory interneuronThe gateTransmission
Large myelinated Aβ (touch, vibration, pressure)Excites itClosesFalls: non-noxious input reduces pain
Small Aδ and C (nociceptive)Inhibits itOpensProceeds
Descending fibres from higher centresModulate itEitherHow attention, mood and expectation reach the cord
  • Explains why rubbing an injury relieves it.
  • The mechanism of transcutaneous electrical nerve stimulation, which selectively stimulates Aβ fibres.

Commonly lost: Many supplementary gate diagrams were drawn inaccurately. Aβ is excitatory to the inhibitory interneuron; C is inhibitory to it. No interneuron means it is not gate control.

2 · The descending inhibitory pathway

LevelStructureMediatorAction
MidbrainPeriaqueductal greyEndogenous opioidsActivated by cortical, hypothalamic and ascending spinoreticular input; rich in opioid receptors
MedullaRostral ventromedial medulla, incl. nucleus raphe magnusSerotoninRelays the descending signal to the cord
PonsLocus coeruleusNoradrenaline (α2)The clonidine and dexmedetomidine target; part of the tricyclic antidepressant mechanism
CordDorsal horn, via the dorsolateral funiculusGABA, glycine, enkephalinsPresynaptic inhibition of transmitter release, postsynaptic hyperpolarisation, and excitation of inhibitory interneurons

Commonly lost: Naming the mediators, noradrenaline and serotonin in particular, carried additional marks beyond describing the anatomy.

3 · Other modifiers

CategoryExamplesDirection
Psychological and cognitiveAttention and distraction, anxiety, depression, expectation, previous experience, cultural context. Placebo analgesia acts on the same brain regions as an opioid, presumably by releasing endogenous opioidsEither
PeripheralSensitisation lowers nociceptor threshold and raises perceived intensityIncreases pain: modification is not only inhibitory
PharmacologicalLocal anaesthetics (conduction block), NSAIDs (prostaglandin-mediated sensitisation), opioids (all three levels), ketamine (NMDA/wind-up), α2 agonists (descending noradrenergic)Reduces pain
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